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egf chemicals, peptides, and recombinant proteins
Egf Chemicals, Peptides, And Recombinant Proteins, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peptide%2C+recombinant+protein+egf/egf+chemicals++peptides++and+recombinant+proteins/pm40239647-597-187-199
Average 90 stars, based on 1 article reviews
Egf Chemicals, Peptides, And Recombinant Proteins, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peptide%2C+recombinant+protein+egf/egf+chemicals++peptides++and+recombinant+proteins/pm40239647-597-187-199
Average 90 stars, based on 1 article reviews
egf chemicals, peptides, and recombinant proteins - by Bioz Stars,
2026-09
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Recombinant:Article Title: Sequentially induced motor neurons from human fibroblasts facilitate locomotor recovery in a rodent spinal cord injury model Article Snippet: Peptide, recombinant protein , SHH , Peprotech , Cat.# 100–45 , 100 ng/ml. .. Article Title: Hair follicle epidermal stem cells define a niche for tactile sensation Article Snippet: Sequence-based reagent , qRT-PCR primers , This paper , , See . .. Article Title: Sequentially induced motor neurons from human fibroblasts facilitate locomotor recovery in a rodent spinal cord injury model Article Snippet: Peptide, recombinant protein , bFGF , Peprotech , Cat.# 100-18B , 20 ng/ml. .. Article Title: Hepatic lipid overload triggers biliary epithelial cell activation via E2Fs Article Snippet: Peptide, recombinant protein , Gastrin , Sigma-Aldrich , G9145 , (10 nM). .. Article Title: Hair follicle epidermal stem cells define a niche for tactile sensation Article Snippet: DOI: https://doi.org/10.7554/eLife.38883 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Rat anti-GFP Nakarai GF090R IHC (1:75) Antibody Mouse anti-laminin g1 Millipore MAB1914 IHC (1:500) Antibody Rat anti-integrin av Abcam RMV-7 IHC (1:500) Antibody Hamster anti-mouse integrin av BD H9.2H8 IHC (1:250) Antibody Goat anti-human integrin av R and D Systems AF1219 IHC (1:100) Antibody Goat anti-mouse integrin a3 R and D Systems AF2787 IHC (1:100) Antibody Hamster anti-mouse integrin b1 BioLegend HMb1-1 IHC (1:100) Antibody Goat anti mouse integrin a8 R and D Systems AF4076 IHC (2.5 mg/ml) Antibody Goat anti-TrkB R and D Systems AF1494 IHC (4 mg/ml) Antibody Rat anti-CD45PE-Cy7 eBioscience 30-F11 FACS (1:100) Antibody Rat anti-TER119-PE-Cy7 eBioscience TER119 FACS (1:100) Antibody Rat anti-CD31PE-Cy7 eBioscience 390 FACS (1:100) Antibody Rat anti-Sca-1PerCP-Cy5.5 eBioscience D7 FACS (1:100) Antibody Rat anti-CD34eFluor660 eBioscience RAM34 FACS (1:100) Antibody Rat anti-CD49fPE eBioscience GoH3 FACS (1:100) Antibody Mouse anti-integrin a2 DSHB P1E6 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a3 DSHB P1B5 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a5 DSHB P1D6 Cell adhesion inhibition (10 mg/ml) Antibody Rat anti-integrin a6 BioLegend GoH3 (313614) IHC (1:500), Cell adhesion inhibition (10 mg/ml) Continued on next page Cheng et al. eLife 2018;7:e38883. .. DOI: https://doi.org/10.7554/eLife.38883 16 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Mouse anti-integrin av ATCC L230 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin b1 DSHB P5D2 Cell adhesion inhibition (10 mg/ml) Recombinant DNA reagent BAC clone containing mouse Egfl6 genomic sequence CHORI BACPAC Resources Center RP23-124O13 Sequence-based reagent qRT-PCR primers This paper See Table 2 other:Article Title: MAPK activity dynamics regulate non-cell autonomous effects of oncogene expression Article Snippet: (species) or resource Designation Source or reference Identifiers Additional information Recombinant DNA reagent pLenti PGKUtr261-EGFP puro This paper pTA152, Regot Lab Recombinant DNA reagent pLenti PGK-P38KTR-mClover This paper pAP50, Regot Lab Recombinant DNA reagent pLenti PGK-JNKKTR-mRuby2 Addgene #59154 PMID:24949979 pSR1846 recombinant DNA reagent lentiCRISPR_ V2_Neo Gift from Dr. Andrew Holland lentiCRISPR_ V2_Puro on addgene as #52961 sequencebased reagent ADAM17KO guide This paper, from IDT 5’- CTACAGATACAT GGGCAGAG-3’ (targets R241 of exon 6) Recombinant DNA reagent pLenti CRISPR ADAM17KO Neo This paper ADAM17KO pTA70, Regot Lab Chemical compound, drug PD-0325901 Selleck Chemicals #S1036 MEKi; MEK inhibitor 5mM Chemical compound, drug Batimastat Selleck Chemicals #S7155 MPi; MMP/ ADAM inhibitor 5mM Chemical compound, drug Gefitinib Selleck Chemicals #S1025 EGFRi; EGFR inhibitor 5mM Chemical compound, drug BIRB-796 Selleck Chemicals # S1574 P38i; p38 inhibitor; BIRB 5mM Chemical compound, drug SB-203580 Sigma # S8307 P38i; p38 inhibitor; SB 25mM Chemical compound, drug SKII Selleck Chemicals #S7176 SKi; Sphingosine Kinase inhibitor 10mM Chemical compound, drug JTE-013 Selleck Chemicals # S128 S1PR2i; S1PR2 inhibitor 10mM Peptide, recombinant protein Doxycycline Sigma #D9891 Dox 2mg/ml Peptide, recombinant protein Amphiregulin Article Title: Oncogene expression from extrachromosomal DNA is driven by copy number amplification and does not require spatial clustering in glioblastoma stem cells Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody mCherry (Rabbit poly- clonal) abcam ab167453 IF (1 in 500) Antibody Rpb1 NTD (D8L4Y) (Rabbit mono- clonal) Cell Signaling Technology #14958 IF (1 in 1000) Antibody Anti- Digoxigenin (Sheep polyclonal) Roche Ref 11333089001 DNA FISH (1 in 10) Antibody Secondary Antibody – Alexa Fluor 647 (Donkey anti- Sheep IgG poly- clonal) Thermo Fisher Scientific A- 21448 DNA FISH (1 in 10) Antibody Secondary Antibody – Alexa Fluor 568 (Donkey anti- Rabbit IgG poly- clonal) Thermo Fisher Scientific A- 10042 IF (1 in 1000) Antibody Secondary Antibody – Alexa Fluor 488 (Donkey anti- Rabbit IgG poly- clonal) Thermo Fisher Scientific A- 21206 IF (1 in 1000) Antibody Secondary Antibody – Alexa Fluor 488 (Donkey anti- Rat IgG poly- clonal) Thermo Fisher Scientific A- 21208 IF (1 in 1000) Genetic reagent (human) Fosmid FISH probe (Human) BACPAC resource https://bacpacresources.org/ library.php?id=275 See Materials and methods - Supplementary file 1 Cell line (Homo sapiens) E20, E25, E26, E28, NSC – GCGR Human Glioma Stem Cells This paper, Glioma Cellular Genetics Resource, CRUK, UK http://gcgr.org.uk; pending publication Other DMEM/HAMS- F12 Sigma- Aldrich Cat#: D8437 Cell culture, media Chemical compound, drug Pen/Strep GIBCO Cat#: 15140–122 Cell culture, media supplement Other BSA Solution GIBCO Cat#: 15260–037 Cell culture, media supplement Other B27 Supplement (×50) LifeTech/GIBCO Cat#: 17504–044 Cell culture, media supplement Other N2 Supplement (×100) LifeTech/GIBCO Cat#: 17502–048 Cell culture, media supplement Other Laminin Cultrex Cat#: 3446- 005- 01 Cell culture, media supplement, and pre- lamination of culture vessels Peptide, Inhibition:Article Title: Hair follicle epidermal stem cells define a niche for tactile sensation Article Snippet: DOI: https://doi.org/10.7554/eLife.38883 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Rat anti-GFP Nakarai GF090R IHC (1:75) Antibody Mouse anti-laminin g1 Millipore MAB1914 IHC (1:500) Antibody Rat anti-integrin av Abcam RMV-7 IHC (1:500) Antibody Hamster anti-mouse integrin av BD H9.2H8 IHC (1:250) Antibody Goat anti-human integrin av R and D Systems AF1219 IHC (1:100) Antibody Goat anti-mouse integrin a3 R and D Systems AF2787 IHC (1:100) Antibody Hamster anti-mouse integrin b1 BioLegend HMb1-1 IHC (1:100) Antibody Goat anti mouse integrin a8 R and D Systems AF4076 IHC (2.5 mg/ml) Antibody Goat anti-TrkB R and D Systems AF1494 IHC (4 mg/ml) Antibody Rat anti-CD45PE-Cy7 eBioscience 30-F11 FACS (1:100) Antibody Rat anti-TER119-PE-Cy7 eBioscience TER119 FACS (1:100) Antibody Rat anti-CD31PE-Cy7 eBioscience 390 FACS (1:100) Antibody Rat anti-Sca-1PerCP-Cy5.5 eBioscience D7 FACS (1:100) Antibody Rat anti-CD34eFluor660 eBioscience RAM34 FACS (1:100) Antibody Rat anti-CD49fPE eBioscience GoH3 FACS (1:100) Antibody Mouse anti-integrin a2 DSHB P1E6 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a3 DSHB P1B5 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a5 DSHB P1D6 Cell adhesion inhibition (10 mg/ml) Antibody Rat anti-integrin a6 BioLegend GoH3 (313614) IHC (1:500), Cell adhesion inhibition (10 mg/ml) Continued on next page Cheng et al. eLife 2018;7:e38883. .. DOI: https://doi.org/10.7554/eLife.38883 16 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Mouse anti-integrin av ATCC L230 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin b1 DSHB P5D2 Cell adhesion inhibition (10 mg/ml) Recombinant DNA reagent BAC clone containing mouse Egfl6 genomic sequence CHORI BACPAC Resources Center RP23-124O13 Sequence-based reagent qRT-PCR primers This paper See Table 2 BAC Assay:Article Title: Hair follicle epidermal stem cells define a niche for tactile sensation Article Snippet: DOI: https://doi.org/10.7554/eLife.38883 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Rat anti-GFP Nakarai GF090R IHC (1:75) Antibody Mouse anti-laminin g1 Millipore MAB1914 IHC (1:500) Antibody Rat anti-integrin av Abcam RMV-7 IHC (1:500) Antibody Hamster anti-mouse integrin av BD H9.2H8 IHC (1:250) Antibody Goat anti-human integrin av R and D Systems AF1219 IHC (1:100) Antibody Goat anti-mouse integrin a3 R and D Systems AF2787 IHC (1:100) Antibody Hamster anti-mouse integrin b1 BioLegend HMb1-1 IHC (1:100) Antibody Goat anti mouse integrin a8 R and D Systems AF4076 IHC (2.5 mg/ml) Antibody Goat anti-TrkB R and D Systems AF1494 IHC (4 mg/ml) Antibody Rat anti-CD45PE-Cy7 eBioscience 30-F11 FACS (1:100) Antibody Rat anti-TER119-PE-Cy7 eBioscience TER119 FACS (1:100) Antibody Rat anti-CD31PE-Cy7 eBioscience 390 FACS (1:100) Antibody Rat anti-Sca-1PerCP-Cy5.5 eBioscience D7 FACS (1:100) Antibody Rat anti-CD34eFluor660 eBioscience RAM34 FACS (1:100) Antibody Rat anti-CD49fPE eBioscience GoH3 FACS (1:100) Antibody Mouse anti-integrin a2 DSHB P1E6 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a3 DSHB P1B5 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a5 DSHB P1D6 Cell adhesion inhibition (10 mg/ml) Antibody Rat anti-integrin a6 BioLegend GoH3 (313614) IHC (1:500), Cell adhesion inhibition (10 mg/ml) Continued on next page Cheng et al. eLife 2018;7:e38883. .. DOI: https://doi.org/10.7554/eLife.38883 16 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Mouse anti-integrin av ATCC L230 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin b1 DSHB P5D2 Cell adhesion inhibition (10 mg/ml) Recombinant DNA reagent BAC clone containing mouse Egfl6 genomic sequence CHORI BACPAC Resources Center RP23-124O13 Sequence-based reagent qRT-PCR primers This paper See Table 2 Sequencing:Article Title: Hair follicle epidermal stem cells define a niche for tactile sensation Article Snippet: DOI: https://doi.org/10.7554/eLife.38883 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Rat anti-GFP Nakarai GF090R IHC (1:75) Antibody Mouse anti-laminin g1 Millipore MAB1914 IHC (1:500) Antibody Rat anti-integrin av Abcam RMV-7 IHC (1:500) Antibody Hamster anti-mouse integrin av BD H9.2H8 IHC (1:250) Antibody Goat anti-human integrin av R and D Systems AF1219 IHC (1:100) Antibody Goat anti-mouse integrin a3 R and D Systems AF2787 IHC (1:100) Antibody Hamster anti-mouse integrin b1 BioLegend HMb1-1 IHC (1:100) Antibody Goat anti mouse integrin a8 R and D Systems AF4076 IHC (2.5 mg/ml) Antibody Goat anti-TrkB R and D Systems AF1494 IHC (4 mg/ml) Antibody Rat anti-CD45PE-Cy7 eBioscience 30-F11 FACS (1:100) Antibody Rat anti-TER119-PE-Cy7 eBioscience TER119 FACS (1:100) Antibody Rat anti-CD31PE-Cy7 eBioscience 390 FACS (1:100) Antibody Rat anti-Sca-1PerCP-Cy5.5 eBioscience D7 FACS (1:100) Antibody Rat anti-CD34eFluor660 eBioscience RAM34 FACS (1:100) Antibody Rat anti-CD49fPE eBioscience GoH3 FACS (1:100) Antibody Mouse anti-integrin a2 DSHB P1E6 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a3 DSHB P1B5 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a5 DSHB P1D6 Cell adhesion inhibition (10 mg/ml) Antibody Rat anti-integrin a6 BioLegend GoH3 (313614) IHC (1:500), Cell adhesion inhibition (10 mg/ml) Continued on next page Cheng et al. eLife 2018;7:e38883. .. DOI: https://doi.org/10.7554/eLife.38883 16 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Mouse anti-integrin av ATCC L230 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin b1 DSHB P5D2 Cell adhesion inhibition (10 mg/ml) Recombinant DNA reagent BAC clone containing mouse Egfl6 genomic sequence CHORI BACPAC Resources Center RP23-124O13 Sequence-based reagent qRT-PCR primers This paper See Table 2 Quantitative RT-PCR:Article Title: Hair follicle epidermal stem cells define a niche for tactile sensation Article Snippet: DOI: https://doi.org/10.7554/eLife.38883 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Rat anti-GFP Nakarai GF090R IHC (1:75) Antibody Mouse anti-laminin g1 Millipore MAB1914 IHC (1:500) Antibody Rat anti-integrin av Abcam RMV-7 IHC (1:500) Antibody Hamster anti-mouse integrin av BD H9.2H8 IHC (1:250) Antibody Goat anti-human integrin av R and D Systems AF1219 IHC (1:100) Antibody Goat anti-mouse integrin a3 R and D Systems AF2787 IHC (1:100) Antibody Hamster anti-mouse integrin b1 BioLegend HMb1-1 IHC (1:100) Antibody Goat anti mouse integrin a8 R and D Systems AF4076 IHC (2.5 mg/ml) Antibody Goat anti-TrkB R and D Systems AF1494 IHC (4 mg/ml) Antibody Rat anti-CD45PE-Cy7 eBioscience 30-F11 FACS (1:100) Antibody Rat anti-TER119-PE-Cy7 eBioscience TER119 FACS (1:100) Antibody Rat anti-CD31PE-Cy7 eBioscience 390 FACS (1:100) Antibody Rat anti-Sca-1PerCP-Cy5.5 eBioscience D7 FACS (1:100) Antibody Rat anti-CD34eFluor660 eBioscience RAM34 FACS (1:100) Antibody Rat anti-CD49fPE eBioscience GoH3 FACS (1:100) Antibody Mouse anti-integrin a2 DSHB P1E6 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a3 DSHB P1B5 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a5 DSHB P1D6 Cell adhesion inhibition (10 mg/ml) Antibody Rat anti-integrin a6 BioLegend GoH3 (313614) IHC (1:500), Cell adhesion inhibition (10 mg/ml) Continued on next page Cheng et al. eLife 2018;7:e38883. .. DOI: https://doi.org/10.7554/eLife.38883 16 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Mouse anti-integrin av ATCC L230 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin b1 DSHB P5D2 Cell adhesion inhibition (10 mg/ml) Recombinant DNA reagent BAC clone containing mouse Egfl6 genomic sequence CHORI BACPAC Resources Center RP23-124O13 Sequence-based reagent qRT-PCR primers This paper See Table 2 Mutagenesis:Article Title: Hair follicle epidermal stem cells define a niche for tactile sensation Article Snippet: DOI: https://doi.org/10.7554/eLife.38883 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Rat anti-GFP Nakarai GF090R IHC (1:75) Antibody Mouse anti-laminin g1 Millipore MAB1914 IHC (1:500) Antibody Rat anti-integrin av Abcam RMV-7 IHC (1:500) Antibody Hamster anti-mouse integrin av BD H9.2H8 IHC (1:250) Antibody Goat anti-human integrin av R and D Systems AF1219 IHC (1:100) Antibody Goat anti-mouse integrin a3 R and D Systems AF2787 IHC (1:100) Antibody Hamster anti-mouse integrin b1 BioLegend HMb1-1 IHC (1:100) Antibody Goat anti mouse integrin a8 R and D Systems AF4076 IHC (2.5 mg/ml) Antibody Goat anti-TrkB R and D Systems AF1494 IHC (4 mg/ml) Antibody Rat anti-CD45PE-Cy7 eBioscience 30-F11 FACS (1:100) Antibody Rat anti-TER119-PE-Cy7 eBioscience TER119 FACS (1:100) Antibody Rat anti-CD31PE-Cy7 eBioscience 390 FACS (1:100) Antibody Rat anti-Sca-1PerCP-Cy5.5 eBioscience D7 FACS (1:100) Antibody Rat anti-CD34eFluor660 eBioscience RAM34 FACS (1:100) Antibody Rat anti-CD49fPE eBioscience GoH3 FACS (1:100) Antibody Mouse anti-integrin a2 DSHB P1E6 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a3 DSHB P1B5 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a5 DSHB P1D6 Cell adhesion inhibition (10 mg/ml) Antibody Rat anti-integrin a6 BioLegend GoH3 (313614) IHC (1:500), Cell adhesion inhibition (10 mg/ml) Continued on next page Cheng et al. eLife 2018;7:e38883. .. DOI: https://doi.org/10.7554/eLife.38883 16 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Mouse anti-integrin av ATCC L230 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin b1 DSHB P5D2 Cell adhesion inhibition (10 mg/ml) Recombinant DNA reagent BAC clone containing mouse Egfl6 genomic sequence CHORI BACPAC Resources Center RP23-124O13 Sequence-based reagent qRT-PCR primers This paper See Table 2 Clinical Proteomics:Article Title: Hair follicle epidermal stem cells define a niche for tactile sensation Article Snippet: DOI: https://doi.org/10.7554/eLife.38883 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Rat anti-GFP Nakarai GF090R IHC (1:75) Antibody Mouse anti-laminin g1 Millipore MAB1914 IHC (1:500) Antibody Rat anti-integrin av Abcam RMV-7 IHC (1:500) Antibody Hamster anti-mouse integrin av BD H9.2H8 IHC (1:250) Antibody Goat anti-human integrin av R and D Systems AF1219 IHC (1:100) Antibody Goat anti-mouse integrin a3 R and D Systems AF2787 IHC (1:100) Antibody Hamster anti-mouse integrin b1 BioLegend HMb1-1 IHC (1:100) Antibody Goat anti mouse integrin a8 R and D Systems AF4076 IHC (2.5 mg/ml) Antibody Goat anti-TrkB R and D Systems AF1494 IHC (4 mg/ml) Antibody Rat anti-CD45PE-Cy7 eBioscience 30-F11 FACS (1:100) Antibody Rat anti-TER119-PE-Cy7 eBioscience TER119 FACS (1:100) Antibody Rat anti-CD31PE-Cy7 eBioscience 390 FACS (1:100) Antibody Rat anti-Sca-1PerCP-Cy5.5 eBioscience D7 FACS (1:100) Antibody Rat anti-CD34eFluor660 eBioscience RAM34 FACS (1:100) Antibody Rat anti-CD49fPE eBioscience GoH3 FACS (1:100) Antibody Mouse anti-integrin a2 DSHB P1E6 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a3 DSHB P1B5 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a5 DSHB P1D6 Cell adhesion inhibition (10 mg/ml) Antibody Rat anti-integrin a6 BioLegend GoH3 (313614) IHC (1:500), Cell adhesion inhibition (10 mg/ml) Continued on next page Cheng et al. eLife 2018;7:e38883. .. DOI: https://doi.org/10.7554/eLife.38883 16 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Mouse anti-integrin av ATCC L230 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin b1 DSHB P5D2 Cell adhesion inhibition (10 mg/ml) Recombinant DNA reagent BAC clone containing mouse Egfl6 genomic sequence CHORI BACPAC Resources Center RP23-124O13 Sequence-based reagent qRT-PCR primers This paper See Table 2 Sample Prep:Article Title: Hair follicle epidermal stem cells define a niche for tactile sensation Article Snippet: DOI: https://doi.org/10.7554/eLife.38883 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Rat anti-GFP Nakarai GF090R IHC (1:75) Antibody Mouse anti-laminin g1 Millipore MAB1914 IHC (1:500) Antibody Rat anti-integrin av Abcam RMV-7 IHC (1:500) Antibody Hamster anti-mouse integrin av BD H9.2H8 IHC (1:250) Antibody Goat anti-human integrin av R and D Systems AF1219 IHC (1:100) Antibody Goat anti-mouse integrin a3 R and D Systems AF2787 IHC (1:100) Antibody Hamster anti-mouse integrin b1 BioLegend HMb1-1 IHC (1:100) Antibody Goat anti mouse integrin a8 R and D Systems AF4076 IHC (2.5 mg/ml) Antibody Goat anti-TrkB R and D Systems AF1494 IHC (4 mg/ml) Antibody Rat anti-CD45PE-Cy7 eBioscience 30-F11 FACS (1:100) Antibody Rat anti-TER119-PE-Cy7 eBioscience TER119 FACS (1:100) Antibody Rat anti-CD31PE-Cy7 eBioscience 390 FACS (1:100) Antibody Rat anti-Sca-1PerCP-Cy5.5 eBioscience D7 FACS (1:100) Antibody Rat anti-CD34eFluor660 eBioscience RAM34 FACS (1:100) Antibody Rat anti-CD49fPE eBioscience GoH3 FACS (1:100) Antibody Mouse anti-integrin a2 DSHB P1E6 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a3 DSHB P1B5 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a5 DSHB P1D6 Cell adhesion inhibition (10 mg/ml) Antibody Rat anti-integrin a6 BioLegend GoH3 (313614) IHC (1:500), Cell adhesion inhibition (10 mg/ml) Continued on next page Cheng et al. eLife 2018;7:e38883. .. DOI: https://doi.org/10.7554/eLife.38883 16 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Mouse anti-integrin av ATCC L230 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin b1 DSHB P5D2 Cell adhesion inhibition (10 mg/ml) Recombinant DNA reagent BAC clone containing mouse Egfl6 genomic sequence CHORI BACPAC Resources Center RP23-124O13 Sequence-based reagent qRT-PCR primers This paper See Table 2 Software:Article Title: Hair follicle epidermal stem cells define a niche for tactile sensation Article Snippet: DOI: https://doi.org/10.7554/eLife.38883 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Rat anti-GFP Nakarai GF090R IHC (1:75) Antibody Mouse anti-laminin g1 Millipore MAB1914 IHC (1:500) Antibody Rat anti-integrin av Abcam RMV-7 IHC (1:500) Antibody Hamster anti-mouse integrin av BD H9.2H8 IHC (1:250) Antibody Goat anti-human integrin av R and D Systems AF1219 IHC (1:100) Antibody Goat anti-mouse integrin a3 R and D Systems AF2787 IHC (1:100) Antibody Hamster anti-mouse integrin b1 BioLegend HMb1-1 IHC (1:100) Antibody Goat anti mouse integrin a8 R and D Systems AF4076 IHC (2.5 mg/ml) Antibody Goat anti-TrkB R and D Systems AF1494 IHC (4 mg/ml) Antibody Rat anti-CD45PE-Cy7 eBioscience 30-F11 FACS (1:100) Antibody Rat anti-TER119-PE-Cy7 eBioscience TER119 FACS (1:100) Antibody Rat anti-CD31PE-Cy7 eBioscience 390 FACS (1:100) Antibody Rat anti-Sca-1PerCP-Cy5.5 eBioscience D7 FACS (1:100) Antibody Rat anti-CD34eFluor660 eBioscience RAM34 FACS (1:100) Antibody Rat anti-CD49fPE eBioscience GoH3 FACS (1:100) Antibody Mouse anti-integrin a2 DSHB P1E6 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a3 DSHB P1B5 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a5 DSHB P1D6 Cell adhesion inhibition (10 mg/ml) Antibody Rat anti-integrin a6 BioLegend GoH3 (313614) IHC (1:500), Cell adhesion inhibition (10 mg/ml) Continued on next page Cheng et al. eLife 2018;7:e38883. .. DOI: https://doi.org/10.7554/eLife.38883 16 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Mouse anti-integrin av ATCC L230 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin b1 DSHB P5D2 Cell adhesion inhibition (10 mg/ml) Recombinant DNA reagent BAC clone containing mouse Egfl6 genomic sequence CHORI BACPAC Resources Center RP23-124O13 Sequence-based reagent qRT-PCR primers This paper See Table 2 Control:Article Title: Hair follicle epidermal stem cells define a niche for tactile sensation Article Snippet: DOI: https://doi.org/10.7554/eLife.38883 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Rat anti-GFP Nakarai GF090R IHC (1:75) Antibody Mouse anti-laminin g1 Millipore MAB1914 IHC (1:500) Antibody Rat anti-integrin av Abcam RMV-7 IHC (1:500) Antibody Hamster anti-mouse integrin av BD H9.2H8 IHC (1:250) Antibody Goat anti-human integrin av R and D Systems AF1219 IHC (1:100) Antibody Goat anti-mouse integrin a3 R and D Systems AF2787 IHC (1:100) Antibody Hamster anti-mouse integrin b1 BioLegend HMb1-1 IHC (1:100) Antibody Goat anti mouse integrin a8 R and D Systems AF4076 IHC (2.5 mg/ml) Antibody Goat anti-TrkB R and D Systems AF1494 IHC (4 mg/ml) Antibody Rat anti-CD45PE-Cy7 eBioscience 30-F11 FACS (1:100) Antibody Rat anti-TER119-PE-Cy7 eBioscience TER119 FACS (1:100) Antibody Rat anti-CD31PE-Cy7 eBioscience 390 FACS (1:100) Antibody Rat anti-Sca-1PerCP-Cy5.5 eBioscience D7 FACS (1:100) Antibody Rat anti-CD34eFluor660 eBioscience RAM34 FACS (1:100) Antibody Rat anti-CD49fPE eBioscience GoH3 FACS (1:100) Antibody Mouse anti-integrin a2 DSHB P1E6 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a3 DSHB P1B5 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin a5 DSHB P1D6 Cell adhesion inhibition (10 mg/ml) Antibody Rat anti-integrin a6 BioLegend GoH3 (313614) IHC (1:500), Cell adhesion inhibition (10 mg/ml) Continued on next page Cheng et al. eLife 2018;7:e38883. .. DOI: https://doi.org/10.7554/eLife.38883 16 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Mouse anti-integrin av ATCC L230 Cell adhesion inhibition (10 mg/ml) Antibody Mouse anti-integrin b1 DSHB P5D2 Cell adhesion inhibition (10 mg/ml) Recombinant DNA reagent BAC clone containing mouse Egfl6 genomic sequence CHORI BACPAC Resources Center RP23-124O13 Sequence-based reagent qRT-PCR primers This paper See Table 2 |
